Targeting Mitochondria for Tomorrow's Health
DRUG SCREENING
The screening and drug discovery strategy at Mitopharma is meticulously designed by utilizing long-lived pharmacology and the latest discovery approaches.
Drug libraries are screened against developed disease models. During drug screening, the generated data is used to detect and identify pharmacological targets that drive mitochondrial dysfunction. Identifying disease targets is one of the major milestones in the drug discovery process.
High Throughput Screening (HTS). Multimode microplate reader with specialized measurement types and capacity of up to 20 (384-well) plates during a single screening. Fast end-point or kinetic read times.
High Content Screening (HCS). Automated HCS systems with the microplate capacity of up to 384-well plates, robotic microplate and liquid handling. Single-cell events can be observed and quantified in live or fixed cells, isolated organs, or small animal models.
HT-Flow cytometry. Instrument with cell sorting capability. Cellular events and phenotypes can be observed and quantified in large number of cells. Cell populations of interest can be sorted and isolated for further culturing or analysis (e.g., gene expression profiling).
Quantitative Polymerase Chain Reaction (qPCR). System for detection and quantitation of changes in gene expression and profiling disease models during drug screening.
HT-RT-PCR. High-throughput (reverse-transcription PCR/PCR) for automated amplification of large quantity of samples. Cell transcriptomics.
HPLC-MS/MS. System for detection and identification of cellular metabolites, proteins, and protein modifications that occur in signaling pathways, as well as ligand-protein and protein-protein interactions. Peptide mapping with High Throughput (HT) sample preparation enables for the parallel analysis and identification of 96 single protein or whole proteome samples.
Electrophysiology and isolated organ perfusion. Systems for the measurement of heart and brain cell function in intact organs or organ slices.
Protein/small molecule crystallography. Protein and small molecule structure determination, structural analysis of ligand interactions. Automated protein crystallization and crystal analysis.