Mitochondrial damage and dysfunction are the primary focus in developed models. Probing and identifying cellular processes elucidate the underlying mechanisms of how mitochondrial damage and dysfunction occur and how they define cell fate.

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Mechanisms are elucidated by examining cellular parameters and events, ranging from gene expression patterns to organelle health analysis, biochemical events, and protein interactions. The puzzle of observed changes and events in cells is assembled into overall mechanisms that shed light on the model behavior and suitability for drug screening.

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Observed model parameters are part of the drug screening pipeline. More than 50 different events and parameters are identified and used for detailed screening in vitro and ex vivo.

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Several strategies of pharmacological modulation and CRISPR-Cas9 gene knock-in and knock-out are employed to achieve the desired disease. Advanced cell culture technologies utilized at Mitopharma allow disease modeling in human and murine continuous cell lines, human iPSCs and differentiated cell lines, as well as murine primary cells.